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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Vet Immunol Immunopathol. 2017 Oct 27;193-194:38–49. doi: 10.1016/j.vetimm.2017.10.004

Figure 7. BCG viability is reduced when cultured with cells from M. bovis-infected animals.

Figure 7

Cells were collected 72 hours after infection with BCG and frozen until ready for analysis. After thawing, cells were re-suspended in 7H9-OADC-T and incubated in for 24 h. Cells were centrifuged and re-suspended in saline solution, and 1 μL of FDA at a concentration of 2 mg/mL was added to each tube. After 30 min of incubation at 37°C, samples were analyzed by flow cytometry. Data represent means ± SEM (n=19 for naïve group and n=8 for infected group) (**** P≤0.0001; ANOVA).