. Author manuscript; available in PMC: 2018 Dec 1.

Published in final edited form as: Vet Immunol Immunopathol. 2017 Oct 27;193-194:38–49. doi: 10.1016/j.vetimm.2017.10.004

Table 4.

Confirming RNA sequencing with qPCR.

Gene	RNA Sequencing	RT-qPCR
IFN-γ	99646.37 (± 69587.71)	2145.75 (± 1994.26)
IL-17	88228.55 (± 52531.11)	1546.28 (± 1343.04)
TNF-α	13.1 (± 3.16)	523.49 (± 493.45)
CCL2	1.06 (± 0.1)	100.79 (±68.69)
CXCL10	80726.06 (± 31605.93)	247.42 (± 196.03)
NOS2	25.99 (± 11.85)	87.38 (± 57.96)
SOCS1	67.11 (± 19.08)	24.16 (± 9.03)
Granzyme B	27526.51 (± 20565.78)	29.8 (± 20.77)

γδ T cells from calves that were subjected to RNA sequencing were also analyzed by qPCR to confirm sequencing results. Values indicate average fold change ± SEM in gene expression between unstimulated and PPD-b stimulated γδ T cells isolated from M. bovis-infected calves (n=5).