Table 5.

Quantitative measure of mRNA labeling using RNAScope for various cytokines and chemokines expressed by γδ T cells within bovine pulmonary tuberculoid granulomas.

	Lung (n=5)	Lymph node (n=5)
IFN-γ	6.94% (± 4%)	22.82% (± 9%)
IL-10	4.68% (± 3%)	16.45% (± 1%)
IL-17	12.00% (± 8%)	12.87% (± 8%)
CCL2	38.22% (± 9%)	21.37% (± 6%)

Tissue samples from granulomatous lesions in the lungs and mediastinal lymph nodes were harvested from calves (n=5) 3 months post-infection with virulent M. bovis. Tissue sections were preserved onto slides by formalin fixation and paraffin embedding. RNAScope was used for in situ analysis of mRNA transcripts of various cytokines/chemokines and the γδ TCR. Ten representative images at 100X magnification from around the periphery of the granuloma were used for quantification. Values represent the percentage of γδ T cells expressing the cytokine or chemokine of interest out of the total number of cells expressing the cytokine or chemokine of interest ± SEM (n=4 for CCL2 in the lung).