Figure 3.

Tissue-specific expression of MT1 genes in rice roots during aerenchyma formation. (A-D) Relative transcript levels of MT1a (A), MT1b (B), MT1c (C), and MT1Ld (D) in the central cylinder (CC), cortex (Co), and outer part of the root (OPR) at 10 mm (± 2 mm) from the root tips under aerated or stagnant conditions for 36 h. The 30 to 50 mm roots of the 20-d-old aerobically grown rice seedlings were subjected to the treatments. The gene encoding transcription initiation factor IIE, TFIIE, was used as a control. Values are means ± SD (n = 3). Significant differences between aerated and stagnant conditions at P < 0.01 and P < 0.05 (two sample t test) are denoted by ** and *, respectively. The methods are described in more detail by Yamauchi and colleagues.¹³