Figure 1.
Transient co-expression of 35Spro:NTA-GFP (green signal) with mCherry-labeled secretory markers (magenta signal) in in tobacco epidermal cells.13 (A) NTA-GFP (green) colocalizes with Golgi (Man49-mCherry) (white indicates overlapping GFP and mCherry signals). (B) NTA-GFP does not colocalize with an Endoplasmic Reticulum marker (SP-mCherry-HDEL). (C) NTA-GFP does not colocalize with peroxisomes (mCherry-PTS1). Four- to Six-week-old Nicotiana benthamiana plants were used in all transient co-expression assays. Agrobacterium tumefaciens (GV3101) harboring the NTA-GFP (or other MLO) and respective marker constructs were co-infiltrated into mature leaves. Imaging was done using a Leica SP8 confocal microscope three days after infiltration similar to our previous study.7 Bars = 10 μm.