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Quantification of LMNA isoform expression levels by droplet digital PCR (ddPCR). (A-D) Levels of LMNA (lamin A), LMNAΔ150, LMNAΔ447, and LMNAΔ297 were measured in wild type (WT), and HGPS fibroblast cDNA samples by droplet digital PCR TaqMan assay. Raw transcript count in copies/ng was normalized to GAPDH. Values represent mean +/− SD derived from Poisson 95% confidence interval.
