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. 2017 Nov 6;114(47):E10161–E10168. doi: 10.1073/pnas.1710680114

Fig. 2.

Fig. 2.

ITGB8 is expressed at higher levels in human Tregs compared with Th cells. (A) The indicated Treg and Th cells were stimulated with anti-CD3/CD28 antibodies during 24 h. Expression of the indicated genes and of housekeeping gene GUSB was analyzed by RT-qPCR. Values correspond to 1,000 × 2∆Ct, where ∆Ct = Ct [GUSB] − Ct [integrin subunit], and therefore represent copies of the indicated mRNA per 1,000 copies of the GUSB mRNA. (B) The indicated Treg and Th cells were stimulated with anti-CD3/CD28 antibodies and collected at various time points. Expression of ITGB8 and housekeeping gene EF-1 were analyzed by RT-qPCR. Values correspond to mRNA copy number as determined by using a standard curve. (C) Polyclonal Treg and Th cells from donor 6 were stimulated with anti-CD3/CD28 antibodies for 24 h. Cell lysates were immunoprecipitated with anti-integrin β8 mAb (mAb 37E1-B5). Total lysates (4% of input in IP) and IP products were analyzed by Western blot with an antibody against integrin αV subunit. Results are representative of three independent experiments.