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. 2017 Nov 6;114(47):E10132–E10141. doi: 10.1073/pnas.1710837114

Fig. 7.

Fig. 7.

RA from pachytene spermatocytes is required for postmeiotic but not premeiotic transitions. (A and B) Percentage of testis tubule cross-sections containing aligned spermatozoa (A) and step 7 and 8 spermatids (B), in controls or 11.6 d after first HU injection. HU + WIN: mice were given injections of HU and then daily injections of WIN18,446, beginning 7.6 d after first HU injection. HU + RA, mice received single dose of RA, 9.6 d after first HU injection. Error bars, mean ± SD, *P < 0.01 (Tukey–Kramer test). NS, not significant (P > 0.05). (C) Percentage of testis tubule cross-sections containing preleptotene spermatocytes, in controls or 11.6 d after first HU injection, and in Dmc1- or Spo11-deficient testes. Error bars, mean ± SD, *P < 0.05, **P < 0.01 (Tukey–Kramer test). NS, not significant (P > 0.05). (D) Immunostaining for STRA8 and KIT on control or HU-injected testis cross-sections. Roman numerals indicate stages. Insets (14.5 μm × 14.5 μm) enlarge boxed regions. Dashed lines, basal laminae of tubule cross-sections. Arrowheads, spermatogonia (white) and preleptotene spermatocytes (yellow). (Scale bar: 10 μm.)