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. 2017 Nov 6;114(47):12596–12601. doi: 10.1073/pnas.1712887114

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Fig. 2. — Arachidonic acid (AA)-containing phospholipids of the splenic white pulp are depleted during F. novicida infection. (A) Image of m/z 885.6 ion channel identified at 1-stearoyl, 2-arachidonyl-phosphatidylinositol (SAPI), [M-H]⁻, 75-μm spatial resolution, MALDI-TOF. Serial sections of spleens are as in Fig. 1A. False color ion intensity is given in red color scale, 0–325 a.u., normalized (TIC). White box outlines white pulp nodule with an active germinal center (identified in black box, SI Appendix, Fig. S1). (B–E) Quantitative LC/MS of parent phospholipid containing arachidonyl group normalized to total input splenic protein content (in grams) evaluated every 12 hours postinfection (h.p.i.), lethal dose F. novicida, SQ. (B) SAPI, m/z 885.6. (C) Palmitoyl, arachidonyl phosphatidylinositol (PI) 16:0/20:4 (PI 36:4), m/z 857.6. (D) Stearoyl, arachidonyl phosphatidylethanolamine (PE) 18:0/20:4 (PE 38:4), m/z 766.5. (E) Stearoyl, arachidonyl phosphatidic acid (PA) 18:0/20:4 (PA 38:4), m/z 723.5. Lipid levels were analyzed using one-way ANOVA for injection time points, *P < 0.05, **P < 0.01, ***P < 0.001 (n = 10). Error bars denote SEM.