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. 2017 Nov 6;114(47):E10216–E10223. doi: 10.1073/pnas.1713897114

Fig. 2.

Fig. 2.

Brief 1-min channelrhodopsin activation of ARNKISS neurons evokes pulsatile LH secretion in freely behaving intact male and female mice. (A) Schematic of experiment. (B) Repeated 1-min blue light (473-nm) activations of ARNKISS neurons evoke LH pulses in an intact male mouse. (C) Mean (±SEM) LH levels (red symbols indicate significantly elevated LH levels; repeated-measures ANOVA with Dunnett’s post hoc tests; n = 5). (D) Repeated 1-min blue light (473-nm) activations of ARNKISS neurons evoke LH pulses in an intact female mouse. (E) Mean (±SEM) LH levels (red symbols indicate significantly elevated LH levels; repeated-measures ANOVA with Dunnett’s post hoc tests; n = 6). (F) Overlaid profiles of endogenous (red) and ChR2-activated (blue) LH pulses in intact male mice. Pulses are normalized for fold increase over baseline; endogenous LH pulses (n = 6) were sampled every 10 min and ChR2-activated LH pulses (n = 15) every 6 min.