Fig 1. Yeast two-hybrid (Y2H) host-pathogen protein-protein interactions.

Using Y2H screens against whole human and murine proteome libraries, we detected 273 unique interactions between 20 Coxiella burnetii and 247 human proteins and 157 unique interactions between 17 C. burnetii and 137 murine proteins. We used these data to construct a single host-pathogen protein interaction network, based on murine/human orthology, containing 415 unique interactions between 25 C. burnetii and 363 human proteins. Green nodes represent C. burnetii proteins, whereas pink and red nodes represent host proteins. Twelve C. burnetii proteins interacted with both hosts, three of which participated in conserved interactions (i.e., they interacted with both human proteins and their murine orthologs; shown as red nodes and connected with thick grey edges).