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. 2017 Nov 27;12(11):e0188387. doi: 10.1371/journal.pone.0188387

Fig 4. IL-27 induces NLRP3 dependent release of IL-1β from monocytes.

Fig 4

THP-1 cells were primed with LPS (10 ng/mL, 6 hours) and incubated over night with IL-27 (100 ng/mL) before stimulation with ATP (3 mM, 1 hour). The NLRP3 inhibitor MCC950 (1μM) was added 30 minutes prior to ATP as indicated. Primary monocytes were incubated with IL-27 over night (100 ng/mL) before stimulation with LPS (10 ng/mL) for 6 hours and ATP (3 mM) for 30 minutes. IL-1β was measured in supernatants from THP-1 cells (A) and primary monocytes (C) with EIA. (B) Caspase-1 activity was measured by bioluminescent luciferase activity in fresh cell-free supernatants from THP-1 cells stimulated as described above, with or without the presence of Ac-YVAD-CHO caspase-1 inhibitor after the manufacturer’s instructions. Results are representative of minimum three experiments and data are presented as mean and SEM. Only LPS-primed samples are shown. ***p<0.001 LPS-stimulated cells, ###p<0.001 versus LPS/ATP-stimulated cells and ¤¤¤p<0.001 versus control cells without caspase-1 inhibitor (B).