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. 2017 Nov 27;12(11):e0188387. doi: 10.1371/journal.pone.0188387

Fig 7. IL-27 increases IL-1β and TNF release from PBMCs.

Fig 7

PBMCs were incubated with IL-27 (100 ng/mL) with our without IL-27 antibody or control antibody (5 μg/mL) over night before stimulation with LPS (10 ng/mL) for 6 hours and ATP (3 mM) for 30 minutes. IL-1β (A) and TNF (B) release was measured in supernatants with EIA. Results are presented as mean and SEM of four (IL-1β) and five (TNF) separate experiments. **p<0.01 and ***p<0.001 versus LPS-stimulated cells, ###p<0.001 versus ATP/LPS-stimulated cells, ¤¤¤p<0.001 versus LPS/ATP/IL-27-stimulated cells (IL-1β) and LPS/IL-27-stimulated cells (TNF).