Fig 6. Interactions between threads and glial cells.

(A) GFAP-positive astrocytes (red) are found surrounding two 6E10-positive plaques in this micrograph, but the astrocytes do not show a similar association with 6E10-positive threads. (B) Similarly, Iba-1-positive microglia are typically found in proximity or contact with plaques in the grey matter but do not appear to interact with threads to the same extent, although 6E10-positive deposits can be found inside some microglia in the white matter (arrowheads). Co-labeling with endosome antibody LAMP-1 (red), Iba-1 (blue) and 6E10 (green) reveals these deposits confined within endosomes inside microglia, which indicates that microglia are taking up amyloid deposits in the white matter tract. Scale bar in A = 50μm; B1 = 100μm.