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. 2017 Nov 27;12(11):e0188218. doi: 10.1371/journal.pone.0188218

Fig 8. Myelinopathy in 5xFAD mouse white matter.

Fig 8

Representative images showing Nile Red labeled spinal cord ventral white matter. 37 week old wild type mouse (A1) sample shows normal appearing myelin staining profile. Very infrequent myelin spheroid (arrow) can be seen in old wild type samples. Myelin staining appears normal in 19 week old 5xFAD mouse spinal cord (A2), but abnormalities are clearly visible at 27 weeks (A3) and are more prevalent in 37 week old 5xFAD mouse samples (A4). For example, myelin spheroid/ thickening (B1 arrowheads) and sphere formation (B2, arrowhead) are observed in a 27 week old 5xFAD mouse sample. Quantitative analysis of myelin abnormalities at 19, 27, and 37 week old 5xFAD mice and 37 week old WT mice (C). Results showed a progressive and significant increase of myelin abnormalities from 19 week to 37 week old 5xFAD. ****, p<0.001, ####, p<0.001. (D) Merging of the Nile Red (green) image with 6E10 (red) and DAPI (blue) images reveals that myelin anomalies are found in contact or close proximity to threads (arrows), but also in the absence of threads (arrowheads). Scale bar in A = 50μm, B = 20μm and D = 100μm.