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. 2017 Nov 27;12(11):e0188593. doi: 10.1371/journal.pone.0188593

Fig 5. Modest improvement of gene editing efficiency with some MS-modified gRNAs was observed using Cas9 mRNA and Cas9 protein in lipid co-transfections.

Fig 5

Unmodified (unmod) and modified guide RNAs were co-transfected with Cas9 mRNA (A and B) or Cas9 protein (C and D). Gene editing efficiencies were estimated for PPIB-targeting guide RNAs in U2OS cells (light blue bars) and HeLa cells (dark blue bars; A and C). Functional gene knockout was quantified using a phenotypic analysis with PSMD7- (light blue bars) or PSMD11- (dark blue bars) targeting guide RNAs in a Ubi-EGFP U2OS cell line (B and D). Cell viability was assessed for each lipid transfection experiment: U2OS cells (dark green boxes) and HeLa cells (light green boxes; A and C) and PSMD7 (dark green boxes) and PSMD11 (light green boxes; B and D). NTC = Non-targeting control. Error bars representative of biological triplicates.