Fig 2. RUE attenuates alcohol-induced hepatic steatosis.

(A) Representative images of boron-dipyrromethene (BODIPY) staining of lipid droplets in the liver tissue (200× magnification); (B) Levels of triglyceride (TG) accumulation in serum and liver tissue. All data are presented as the mean ± SEM. (*P < 0.05 vs. control); (C) Immunoblot analyses of lipogenic genes (SREBP-1, ACC, and FAS) in liver tissue with the loading control CyPB. All data are presented as the mean ± SEM. (*P < 0.05 vs. control); (D) Immunoblot analyses of fatty acid oxidative genes (SIRT1, AMPK-α, PGC1-α, and CPT1) with the loading control CyPB. All data are presented as the mean ± SEM. (*P < 0.05 vs. control) ¹RQ, relative quantity.