Fig 8. Fluorescent signal of dyes in simulated emission windows upon different excitation wavelengths.

Each emission window is imitated by integrating the acquired emission spectra only in the wavelength area determined by the commercial optical bandpass filters. The Edmund Optics filter kits are a standard set of filters for 1) FITC, 2) Texas Red, and 3) Cy5, with transmission wavelength ranges of the emission bandpass filters at 539–556 nm, 604–644 nm, and 672–712 nm, respectively. The Chroma laser bandpass filter sets for 4) FITC, 5) mCherry, and 6) ATTO647N had wavelength ranges of 539–550 nm, 593–667 nm, and 669–741 nm, respectively. The background from PVA-sample without dyes was subtracted. The dashed lines are guides for the eye at intensity of 5000 a.u. Error bars represent the standard deviation of two separate pixels in each image.