. 2017 Nov 27;12(11):e0188588. doi: 10.1371/journal.pone.0188588

Table 1. Coefficient of determination (R²) values corresponding to antibodies, concentrations and condition.

Antibody	Dilution	R² value	Antibody	Dilution/condition	R² value
Primary	1:500	0.97	Secondary	1: 10,000	0.99
	1:600	0.99		1:20,000	0.96
	1:700	0.97		1:20,000 + 2.5% BSA	0.97
	1:800	0.97		1:20,000 + 5% BSA	0.98
	1:1000	0.98

Table 1. Coefficient of determination (R2) values corresponding to antibodies, concentrations and condition.