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. 2017 Nov 13;13(11):e1006724. doi: 10.1371/journal.ppat.1006724

Fig 2. OsGLIP1 and OsGLIP2 exhibit lipase activities.

Fig 2

(A) Alignment of OsGLIP1 and OsGLIP2 amino acid sequences with functionally known homologues from Arabidopsis and Tanacetum cinerariifolium. Sequences were aligned using Genedoc. (B) Expression and purification of recombinant OsGLIP1-GST and OsGLIP2-GST proteins in E. coli. (C, D) Lipase activities of OsGLIP1 and OsGLIP2. OsGLIP1 and OsGLIP2 were incubated with p-nitrophenyl acetate (C) and p-nitrophenyl butyrate (D) at 30°C. The absorbance readings were collected every 5 minutes in a time course of 60 min or 120 min. The substrates were incubated with either GST or no protein as controls. Data are shown as means ± SD (n = 3).