Fig 3. Validation of model predictions against experimental observations not used for model reconstruction.

Qualitative activity changes of network nodes were predicted by simulating the response to stretch alone or to stretch together with inhibition of various nodes (first column), and then compared with published experimental observations of in vitro rat cardiomyocytes (second column). A validation threshold of 5% relative change was used. Input-output and input-intermediate activity changes are defined relative to no stretch activity, while inhibition activity changes are defined relative to steady-state stretch activation.