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. 2017 Nov 15;6(11):1680–1691. doi: 10.1242/bio.026278

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© 2017. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 2. — IFT25 and IFT27 form an IFT-A- and IFT-B-independent heterodimer in vivo. Ni-NTA resin elute from whole cell lysates of the IFT25::HA::6×His (A) and IFT27::HA::6×His (B) transgenic strains were fractionated by size exclusion chromatography (S200 sizing column). As detected by western blotting, IFT25::HA::6×His and IFT27 cofractionated at ∼60 kDa, independent of IFT-A protein IFT139 and IFT-B protein IFT46 (A) and IFT27::HA::6×His and IFT25 also cofractionated at ∼60 kDa, independent of IFT139 and IFT46 (B). For both panels, relative total protein concentration of the fractions is shown as absorbance at 280 nm. The elution volume of one standard marker (60 kDa) is indicated on the western blots. WCE, whole cell extracts.