Table 2. Prediction of BF signals for conditions used in tomographic data collection.
From these data, we obtain the predicted intensity ratios between ribosomes and water, ribosomal RNA and water, and TCP and water.
| Scattering cross-sections per atom (>5 mrad) | |
|---|---|
| H* | - |
| C | 0.0042 |
| N | 0.0047 |
| O | 0.0051 |
| P | 0.0165 |
| Mg | 0.0097 |
| S | 0.0182 |
| Ca | 0.0278 |
| Estimated scattering signal per nm3 material | |
| Water | 0.159 |
| Ribosome | 0.249 |
| ribosomal RNA | 0.364 |
| TCP | 0.963 |
| Predicted scattering intensity ratios | |
| Ribosome/water | 1.57 |
| RNA/water | 2.29 |
| TCP/water | 6.06 |
* Hydrogen does not scatter electrons above the cutoff angle for the BF detector.