Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
||
|---|---|---|---|---|---|---|
| Organism, C57BL/6N (Mus musculus) |
TwnkLoxP/LoxP; Twnk L/L |
Milenkovic et al., 2013 | RRID: MGI:5496889 | |||
| Organism, C57BL/6N (Mus musculus) |
TwnkLoxP/LoxP, +/Ckmm-cre; Twnk L/L, cre |
Milenkovic et al., 2013 | RRID: MGI:5496891 | |||
| Organism, C57BL/6N (Mus musculus) |
TfamLoxP/LoxP; Tfam L/L |
Larsson et al., 1998 | RRID: MGI:2177633 | |||
| Organism, C57BL/6N (Mus musculus) |
TfamLoxP/LoxP, +/Ckmm-cre; Tfam L/L, cre |
Larsson et al., 1998 | RRID: MGI:2177634 | |||
| Organism, C57BL/6N (Mus musculus) | PolrmtLoxP/LoxP; Polrmt L/L | Kühl et al., 2014, 2016 | MGI:5704129 | |||
| Organism, C57BL/6N (Mus musculus) | PolrmtLoxP/LoxP, +/Ckmm-cre; Polrmt L/L, cre | Kühl et al., 2014, 2016 | RRID: MGI:5704131 | |||
| Organism, C57BL/6N (Mus musculus) | LrpprcLoxP/LoxP; Lrpprc L/L | Ruzzenente et al., 2012 | RRID: MGI:5438915 | |||
| Organism, C57BL/6N (Mus musculus) | LrpprcLoxP/LoxP, +/Ckmm-cre;Lrpprc L/L, cre | Ruzzenente et al., 2012 | RRID: MGI:5438914 | |||
| Organism, C57BL/6N (Mus musculus) | Mterf4LoxP/LoxP; Mterf4 L/L | Cámara et al., 2011 | RRID: MGI:5288508 | |||
| Organism, C57BL/6N (Mus musculus) | Mterf4LoxP/LoxP, +/Ckmm-cre;Mterf4 L/L, cre | Cámara et al., 2011 | RRID: MGI:5292478 | |||
| Antibody | ALDH18A1 | Thermofisher Scientific | Cat#PA5-19392 RRID: AB_10985670 |
(1:200) | ||
| Antibody | CLPP | Sigma-Aldrich | Cat#WH0008192M1 RRID: AB_1840782 |
(1:300) | ||
| Antibody | COX4 | Cell Signaling | Cat#4850 RRID: AB_2085424 |
(1:500) | ||
| Antibody | CS | Abcam | Cat#ab129095 RRID: AB_11143209 |
(1:200) | ||
| Antibody | FDPS | Abcam | Cat#ab189874 RRID: AB_2716301 |
(1:500) | ||
| Antibody | GLS | Abcam | Cat#ab93434 RRID: AB_10561964 |
(1:200) | ||
| Antibody | HMGCS1 | Abcam | Cat#ab194971 RRID: AB_2716299 |
(1:500) | ||
| Antibody | HSPA9/mtHSP70/Grp75 | Abcam | Cat#ab82591 RRID: AB_1860633 |
(1:200) | ||
| Antibody | LONP1 | Abcam | Cat#ab103809 RRID: AB_10858161 |
(1:500) | ||
| Antibody | LRPPRC mouse | N.-G. Larsson; Ruzzenente et al., 2012 | RRID: AB_2716302 | (1:1000) | ||
| Antibody | MRPL37 | Sigma-Aldrich | Cat#HPA025826 RRID: AB_1854106 |
(1:500) | ||
| Antibody | MRLP44 | Proteintech | Cat#16394–1-AP RRID: AB_2146062 |
(1:300) | ||
| Antibody | MRPS35 | Proteintech | Cat#16457–1-AP RRID: AB_2146521 |
(1:500) | ||
| Antibody | MTHFD1 | Abcam | Cat#ab103698 RRID: AB_10862775 |
(1:500) | ||
| Antibody | MTHFD2 | Abcam | Cat#ab37840 RRID: AB_776544 |
(1:500) | ||
| Antibody | NDUFA9 | Abcam | Cat#ab14713 RRID: AB_301431 |
(1:500) | ||
| Antibody | POLRMT mouse | N.-G. Larsson;Kühl et al., 2014 | RRID: AB_2716297 | |||
| Antibody | PYCR1 | Proteintech | Cat#13108–1-AP RRID: AB_2174878 |
(1:200) | ||
| Antibody | SDHA | Thermofisher Scientific | Cat#459200 RRID: AB_2532231 |
(1:100) | ||
| Antibody | SHMT2 | Sigma-Aldrich | Cat#HPA020543 RRID: AB_1856833 |
(1:500) | ||
| Antibody | TFAM | Abcam | Cat#ab131607 RRID: AB_11154693 |
(1:500) | ||
| Antibody | Total OXPHOS Rodent WB Antibody Cocktail | Abcam | Cat#ab110413 RRID: AB_2629281 |
(1:1000) | ||
| Antibody | Tubulin | Cell Signaling | Cat#2125 RRID: AB_2619646 |
(1:1000) | ||
| Antibody | TWINKLE mouse | N.-G. Larsson,Milenkovic et al., 2013 | RRID: AB_2716298 | |||
| Antibody | UQCRFS1 | Abcam | Cat#ab131152 RRID:AB_2716303 |
(1:200) | ||
| Antibody | VDAC1 | Millipore | Cat#MABN504 RRID:AB_2716304 |
(1:1000) | ||
| Sequence-based reagent | Taqman Assay - Mouse Adck3 | Life technologies | Mm00469737_m1 | |||
| Sequence-based reagent | Taqman Assay - Mouse Adck4 | Life technologies | Mm00505363_m1 | |||
| Sequence-based reagent | Taqman Assay - Mouse Atf4 | Life technologies | Mm00515325_m1 | |||
| Sequence-based reagent | Taqman Assay - Mouse Myc | Life technologies | Mm00487804_m1 | |||
| Sequence-based reagent | Taqman Assay - Mouse Coq2 | Life technologies | Mm01203260_g1 | |||
| Sequence-based reagent | Taqman Assay - Mouse Coq4 | Life technologies | Mm00618552_m1 | |||
| Sequence-based reagent | Taqman Assay - Mouse Coq5 | Life technologies | Mm00518239_m1 | |||
| Sequence-based reagent | Taqman Assay - Mouse Coq7 | Life technologies | Mm00501587_m1 | |||
| Sequence-based reagent | Taqman Assay - Mouse Fdps | Life technologies | Mm00836315_g1 | |||
| Sequence-based reagent | Taqman Assay - Mouse Gabpa | Life technologies | Mm00484598_m1 | |||
| Sequence-based reagent | Taqman Assay - Mouse Hmgcs1 | Life technologies | Mm01304569_m1 | |||
| Sequence-based reagent | Taqman Assay - Mouse Hmgcr | Life technologies | Mm01282499_m1 | |||
| Sequence-based reagent | Taqman Assay - Mouse Mterf4 | Life technologies | Mm00508298_m1 | |||
| Sequence-based reagent | Taqman Assay -Mouse Nrf1 | Life technologies | Mm00447996_m1 | |||
| Sequence-based reagent | Taqman Assay - Mouse Pdss1 | Life technologies | Mm00450958_m1 | |||
| Sequence-based reagent | Taqman Assay - Mouse Pdss2 | Life technologies | Mm01190168_m1 | |||
| Sequence-based reagent | Taqman Assay - Mouse Ppargc1 | Life technologies | Mm00447183_m1 | |||
| Sequence-based reagent | Taqman Assay - Mouse Polrmt | Life technologies | Mm00553272_m1 | |||
| Sequence-based reagent | Taqman Assay - Mouse Tfam | Life technologies | Mm00627275_m1 | |||
| Sequence-based reagent | Taqman Assay - Mouse Peo1/Twnk | Life technologies | Mm00467928_m1 | |||
| Commercial assay or kit | miRNeasy Mini kit | Qiagen | Cat#217004 | |||
| Commercial assay or kit | Ribo-Zero rRNA removal kit | Illumina | MRZH11124 | |||
| Commercial assay or kit | Tru-Seq Sample preparation | Illumina | RS-122–2002 | |||
| Commercial assay or kit | High Capacity cDNA revese transcription kit | Applied Biosystems | Cat#4368814 | |||
| Commercial assay or kit | TaqMan Universal PCR Master Mix, No Amperase UNG | Applied Biosystems | Cat#4324020 | |||
| Commercial assay or kit | Citrate Synthase Assay Kit | Sigma-Aldrich | Cat#CS0720 | |||
| Chemical compound, drug | EDTA-free complete protease inhibitor cocktail | Roche | Cat#05056489001 | |||
| Chemical compound, drug | PhosSTOP tablets | Roche | Cat#04906837001 | |||
| Chemical compound, drug | Percoll | GE Heathcare | Cat#17-0891-02 | |||
| Chemical compound, drug | Trypsin gold | Promega | Cat#V5280 | |||
| Chemical compound, drug | Standard Coenzyme Q9 | Sigma-Aldrich | Cat#27597 | |||
| Chemical compound, drug | Standard Coenzyme Q10 | Sigma-Aldrich | Cat#C9538 | |||
| Chemical compound, drug | Standard Glutamate (Glutamic acid) | Sigma-Aldrich | Cat#G1251 | |||
| Chemical compound, drug | Standard Glycine | Sigma-Aldrich | Cat#G7126 | |||
| Chemical compound, drug | Standard Proline | Sigma-Aldrich | Cat#P0380 | |||
| Chemical compound, drug | Standard Sarcosine | Sigma-Aldrich | Cat#S7672 | |||
| Chemical compound, drug | Standard Serine | Sigma-Aldrich | Cat#S4500 | |||
| Software, algorithm | Cytoscape v. 3.5.0 | Shannon et al., 2003 |
http://www.cytoscape.org
RRID:SCR_003032 |
|||
| Software, algorithm | DESeq2 package R v. 3.3.2 | Other | Love et al. (2014) | |||
| Software, algorithm | Ingenuity Pathway Analysis - Ingenuity Systems | Qiagen |
www.ingenuity.com
RRID:SCR_008653 |
|||
| Software, algorithm | iRegulon v. 1.3 | Janky et al., 2014 | http://iregulon.aertslab.org/download.html | |||
| Software, algorithm | MaxQuant v. 1.5.2.8 | Cox and Mann, 2008 |
http://www.coxdocs.org/doku.php id=maxquant:start
RRID:SCR_014485 |
|||
| Software, algorithm | R - The R project for Statistical Computing |
https://www.r-project.org
RRID:SCR_001905 |
||||
| Software, algorithm | Perseus | Cox and Mann, 2012 | http://www.coxdocs.org/doku.php?id=perseus:start | |||
| Software, algorithm | TargetP v. 1.1 |
Emanuelsson et al., 2000; Nielsen et al., 1997
|
http://www.cbs.dtu.dk/services/TargetP/ | |||
| Other | 1.9 mm ReproSil-Pur 120 C18-AQ media | Dr. Maisch | Cat#r119.aq | |||
| Other | 25 cm, (75 mm internal diameter) PicoFrit analytical column | New Objective | Cat#PF7508250 | |||