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. 2017 Nov 23;8:870. doi: 10.3389/fphar.2017.00870

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Copyright © 2017 Chen, Wang, Qi, Ge, Tian, Li, Zhang, Wang, Huang and Tang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Rhaponticum uniflorum ethyl acetate (RUEA) extracts inhibited cell proliferation and induced apoptosis in SCC15 cells. (A) Morphological changes in SCC15 cells. (B) CCK8 assay of the effect of RUEA extracts on SCC15 cells for 24, 48, and 72 h at concentrations of 0 (vehicle control), 12.5, 25, 50, and 100 μg/mL. (C) Annexin V-FITC/PI double-staining for detection of apoptosis following treatment with RUEA extracts (0, 12.5, 25, 50, and 100 μg/mL) for 24, 48, and 72 h in SCC15 cells. Data representing three independent experiments are shown as means ± SDs. ^∗P < 0.05; ^∗∗P < 0.01.