Table 1.
Resources for zebrafish CRISPR/Cas9 experimental design.
| Resource | Description | Citations |
|---|---|---|
| A Streamlined CRISPR Pipeline to Reliably Generate Zebrafish Frameshifting Alleles | Protocol | Talbot and Amacher, 2014 |
| CRISPR/Cas9-mediated conversion of eGFP- into Gal4-transgenic lines in zebrafish | Protocol | Auer et al., 2014 |
| CRISPRscan (http://www.crisprscan.org/) | sgRNA design tool | Moreno-Mateos et al., 2015 |
| CRISPRz (https://research.nhgri.nih.gov/CRISPRz/) | Zebrafish sgRNA database | Varshney et al., 2016 |
| Codon-optimized Cas9 (Addgene: 64237) | Plasmid | Yin et al., 2015 |
| Vector system for tissue-specific gene disruption (Addgene: 63154, 63155, 63156, 63157) | Plasmids | Ablain et al., 2015 |
| 2C-Cas9 tool (Addgene: 74009, 74010) | Plasmids | Di Donato et al., 2016 |
| Tg(ubb:NLS-zCas9-NLS, myl7:EGFP)vu602 | Transgenic lines | Yin et al., 2015 |
| Tg(actb2:NLS-zCas9-NLS, cryaa:TagRFP)vu603 | ||
| Tg(fabp10a:NLS-zCas9-NLS, myl7:EGFP)vu604 | ||
| Tg(hsp70l:LOXP-mCherry-LOXP-NLS-zCas9-NLS)vu605 |