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. 2017 Nov 27;7:16361. doi: 10.1038/s41598-017-16370-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of T cell development in WT and Snx27 ^−/− mice. (A) WT and Snx27 ^−/− mice littermate-matched pairs of mice were sacrificed and total cellularity recorded of the thymus, spleen and lymph nodes. Data shown as mean ± SEM (*p < 0.05; paired t-test; n = 7). (B) Spleen from WT and Snx27 ^−/− mice littermate-matched pairs are shown for size comparation. (C,D) Thymocytes from WT and Snx27 ^−/− littermates pairs were stained for CD4 and CD8 and analyzed by flow cytometry. (C) Representative flow cytometry plots. (D) The percentage of total double-positive (DP, CD4+ CD8+) and single positive (SP, CD4+ CD8− or CD4−CD8+) thymocytes was calculated. Data shown as mean ± SEM (*p < 0.05; paired t-test; n = 7).