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. 2017 Nov 27;7:16406. doi: 10.1038/s41598-017-16618-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression analysis of Naglu and Hsd17b1. Quantitative RT-PCR (A) analysis of the testis, epididymis, ovary, uterus, kidney, liver, adrenal gland, gonadal white adipose tissue (gWAT) and brown adipose tissue (BAT) showed the downregulation of Naglu expression in the Hsd17b1-LacZ/Neo mice (white bars), Hsd17b1-LacZ mice (dark grey bars) and Hsd17b1-LacZ/Neo X HSD17B1TG mice (light grey bars) compared with WT mice (black bars) at 3 months of age. (B) The Hsd17b1 mRNA expression was not altered in Naglu-Neo mice ovaries. Bars demonstrate the mean of the relative expression normalised to the expression of L19 (the error bars show SD, n = 6). (C) The expression of Naglu was downregulated in the BAC vector with the entire Hsd17b1 gene deleted (expression normalised to luciferase gene expression and error bars show SEM, n = 6). (D) Naglu activity was proven to be disrupted in the livers of Naglu-Neo, Hsd17b1-LacZ/Neo and Hsd17b1-LacZ/Neo X HSD17B1TG mice. Bars demonstrate the mean activity (Error bars SEM, n = 3–5). ND = not determined, NM = not measurable.