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. 2017 Nov 27;7:16373. doi: 10.1038/s41598-017-16693-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The protective effect of minocycline (4 μM) on radiation-induced apoptosis in primary neurons assayed by TUNEL analysis. (a) Bright field images of cultured primary neurons, 60× . (b) Field images of DAPI (blue) and TUNEL (green) staining of cultured primary neurons from different groups. (c) Quantitative analysis of radiation-induced neural apoptosis in different groups at 24 and 48 h post irradiation. **P < 0.01 and ***P < 0.001 compared with the relative control.