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. 2017 Nov 27;7:16329. doi: 10.1038/s41598-017-16426-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Calculation of R₀, a ruler for LRET experiments, in the pair of Tb³⁺ ion bound to LBT and Ts1-S14R-Bodipy. (a) Analytical LCMS data for Ts1-S14R-Bodipy; Calc. 7278.1 Da (average isotopes), Obsd. 7278.4 ± 0.3 Da. (b) Overlap between the emission spectrum of Tb³⁺ bound to LBT (black solid line) and the absorption spectra of Ts1-S14R-Bodipy (blue solid line). The emission spectrum of Ts1-S14R-Bodipy is also shown (green solid line). (c) Energy transfer efficiency as a function of distance.