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. 2017 Nov 27;7:16329. doi: 10.1038/s41598-017-16426-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Distance measurements between Nav-LBTs and Ts1-Bodipy or Ts1-S14R-Bodipy by LRET. (a) Representative raw traces of donor signal (D: blue), donor in presence of acceptor signal (DA: red) and sensitized emission signal (SE: green) of Nav-LBTs and Ts1-Bodipy (left column, DI-3GLBT-R(-5): n = 5; DIII-3GLBT-R(-5): n = 6; and, DIV-3GLBT-R(-5): n = 4; adapted from Kubota T. et al.²¹), or Ts1-S14R-Bodipy (right column, DI-3GLBT-R(-5): n = 8; DIII-3GLBT-R(-5): n = 6: and, DIV-3GLBT-R(-5): n = 5). Time constants are shown as average values ± SEM. (b) Schematic structural map of the location of Ts1-Bodipy (red circle) and Ts1-S14R-Bodipy (blue circle) in Nav1.4, based on LRET measurements. Distance values of Ts1-Bodipy were adopted from Kubota T. et al.²¹. Distances are average values ± SEM calculated from the time constants.