Fig. 3.

Structure and conformational changes. a Global architecture of Kod-RI bound to a P/T duplex. Front (left) and back (right) views of Kod-RI colored by domain with the template and primer strands shown in red and blue, respectively. Structural changes observed during primer–template binding b, nucleoside triphosphate binding c, and translocation d. The 2′-deoxy threose adenosine (tA^d) residue at the 3′-terminus of the DNA primer is colored in orange to distinguish it from the DNA portion of the P/T duplex (yellow sticks). Magnesium ions appear as green spheres. In the translocated structure d, the TNA residues are colored blue