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. 2017 Nov 23;8:2003. doi: 10.3389/fpls.2017.02003

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Copyright © 2017 Arthikala, Montiel, Sánchez-López, Nava, Cárdenas and Quinto.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Superoxide accumulation in Rhizobium-inoculated transgenic roots. Uninoculated and R. tropici-inoculated (72 hpi) transgenic hairy roots were used to estimate superoxide accumulation. (A) Quantity of NBT/formazan precipitation in uninoculated and Rhizobium-inoculated PvRbohA-RNAi roots were biochemically estimated. (B) Quantitative RT-PCR analysis of PvSOD and PvCAT in transgenic PvRbohA-RNAi and control roots. Transcript accumulation was normalized to the expression of the EF1α and IDE reference genes. Values represent the averages of three biological replicates (n > 9). The statistical significance of differences between uninoculated and R. tropici inoculated samples was determined using an unpaired two-tailed Student’s t-test (^∗P < 0.05; ^∗∗P < 0.01). Error bars represent means ± SEM.