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. 2017 Nov 23;8:2003. doi: 10.3389/fpls.2017.02003

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Copyright © 2017 Arthikala, Montiel, Sánchez-López, Nava, Cárdenas and Quinto.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Transcript accumulation pattern of AUX1, cyclins, and polyamine synthesis genes in rhizobia-infected PvRbohA-RNAi roots at 7 dpi. (A) Transcript levels (normalized to uninoculated roots) of P. vulgaris AUX1, (B) CYCB1-1, CYCD1, and CYCD3 cyclin genes, and (C) ADC and ODC polyamine synthesis genes in control and PvRbohA-RNAi roots. Transcript accumulation was normalized to the expression of the EF1α and IDE reference genes. Values represent the averages of three biological replicates (n > 9). The statistical significance of differences between control and PvRbohA-RNAi samples was determined using an unpaired two-tailed Student’s t-test (^∗P < 0.05; ^∗∗P < 0.01). Error bars represent means ± SEM. dpi, days post-inoculation; ADC, arginine decarboxylase; ODC, ornithine decarboxylase.