TABLE 10.
Primers and probes used in the current study
| Purpose and primer | Sequence (5′–3′) | Reference(s) |
|---|---|---|
| 16S sequencing | 95 | |
| 27F | GAGTTTGATCCTGGCTCAG | |
| 1525R | AGAAAGGAGGTGATCCAGCC | |
| gyrB sequencing | 37 | |
| GyrB630F | GGATAACGCGATTGACGAAG | |
| GyrB1245R | ATCRTCYTTCATGGTCGARA | |
| GyrB2198F | TAAAGACGATGAGGCGATGG | |
| GyrB2540R | GCCGTGARCAAARTCRAA | |
| sodB sequencing | 32 | |
| E1F | ATGTCRTTCGAATTACCTGC | |
| 497R | TCGATGTARTARGCGTGTTCCCA | |
| Repetitive sequence-mediated PCR | ||
| BOX | CTACGGCAAGGCGACGCTGACG | 87 |
| ERIC I | ATGTAAGCTCCTGGGGATTCAC | 87 |
| ERIC II | AAGTAAGTGACTGGGGTGAGCG | 59 |
| GTG5 | GTGGTGGTGGTGGTG | 59 |
| Multiplex real-time PCR | ||
| E. tarda | 89 | |
| ET3518F | CAGTGATAAAAAGGGGTGGA | |
| ET3632R | CTACACAGCAACGACAACG | |
| ET3559P | AGACAACAGAGGACGGATGTGGC | |
| E. piscicida | 89 | |
| EP14529F | CTTTGATCATGGTTGCGGAA | |
| EP14659R | CGGCGTTTTCTTTTCTCG | |
| EP14615P | CCGACTCCGCGCAGATAACG | |
| E. anguillarum | 89 | |
| EA1583F | GATCGGGTACGCTGTCAT | |
| EA1708R | AATTGCTCTATACGCACGC | |
| EA1611P | CCCGTGGCTAAATAGGACGCG | |
| E. ictaluri | 70, 96 | |
| EI481F | ACTTATCGCCCTCGCAACTC | |
| EI658R | CCTCTGATAAGTGGTTCTCG | |
| EI561P | CCTCACATATTGCTTCAGCGTCGAC |