TABLE 8.
Specificity of the mPCR assay for each respective targeta
| Species | Mean (SD) Cq |
||||
|---|---|---|---|---|---|
| gDNA alone from species: |
All Edwardsiella gDNA mixed together | ||||
| E. anguillarum | E. ictaluri | E. piscicida | E. tarda | ||
| E. anguillarum | 23.28 (0.07) | — | — | — | 23.21 (0.09) |
| E. ictaluri | — | 22.61 (0.10) | — | — | 22.49 (0.05) |
| E. piscicida | — | — | 22.63 (0.15) | — | 22.50 (0.09) |
| E. tarda | — | — | — | 22.93 (0.13) | 22.50 (0.12) |
a
The user-defined fluorescence threshold for Cq determination was set at 50 relative fluorescence units. Dashes indicate no amplification of DNA.