FIG 3.

NGS analysis results generated from FluLINE. Influenza virus samples processed at WHOCC-Melbourne were sequenced on the Ion Torrent PGM platform, and data were analyzed using the newly developed FluLINE pipeline described in this study. NGS sequencing coverage and depth for representative influenza A virus (A) and influenza B virus (B) are shown. The outermost circle represents the gene segments: HA (orange), NA (blue), and MP (purple). The depths of coverage are represented by the concentric rings, emanating from the center, and each concentric ring represents 1,000× coverage.