TABLE 1.
Universal FluA/B primer cocktail
| Primer | Sequence (5′ to 3′)a | Amt (μl) from each 10 μM oligonucleotideb |
|---|---|---|
| A-HA-UniF | GGGGGGAGCAAAAGCAGGGGA | 40 |
| A-HA-UniR | CCGGGTTATTAGTAGAAACAAGGGTG | 40 |
| A-NA-UniF | GGGGGGAGCAAAAGCAGGAGT | 60 |
| A-NA-UniR | CCGGGTTATTAGTAGAAACAAGGAGT | 60 |
| A-M-UniFc | GGGGGGAGCAAAAGCAGGTAG | 20 |
| A-M-UniRc | CCGGGTTATTAGTAGAAACAAGGTAG | 20 |
| B-HANA-Uni3F | GGGGGAGCAGAAGCAGAGC | 80 |
| B-HANA-Uni3R | CCGGGATATTAGTAGTAACAAGAGC | 80 |
a
Underlined nucleotides correspond to the conserved genomic termini.
b
For highest RT-PCR efficiency, the working stock should be made fresh each time.
c
The primers for the M segment could be left out of the cocktail if the M segment is not needed for the identification of antiviral resistant mutations.