TABLE 2.
Summary of influenza A and B samples amplified and sequenced in this study
| Sequencing platform | Sample format | Virus type/subtype/lineage | No. of successful samples/total no. of samples (%)a |
|---|---|---|---|
| Ion Torrent PGM | Cultured specimens | A(H1N1)pdm09 | 152/152 (100) |
| A(H3N2) | 1,502/1,502 (100) | ||
| B/Yamagata | 61/61 (100) | ||
| B/Victoria | 46/46 (100) | ||
| Ion Torrent PGM; Illumina MiSeq | Primary specimens | A(H1N1)pdm09b | 68/83 (82) |
| A(H3N2) | 158/189 (84) | ||
| B/Yamagata | 10/11 (91) | ||
| B/Victoria | 14/17 (82) | ||
| B/lineage-not-performed | 36/44 (82) |
a
Successful samples are either evidenced by RT-PCR amplification of all desired amplicons (for FluA, HA, NA, and M; for FluB, HA and NA) or by sequencing of complete target genes (for FluA, HA, NA, and M; for FluB, HA and NA).
b
Two of the samples were coinfected with A(H1N1)pdm09 and A(H3N2), or with A(H1N1)pdm09 and influenza B virus.