TABLE 1.
KPNIH1 reference genome coverage by MinION and Illumina readsa
| KPNIH1 chromosome and plasmids |
Plasmid DNA |
Total DNA Illumina |
|||||
|---|---|---|---|---|---|---|---|
| MinION |
Illumina |
||||||
| Genome component | Size (kb) | No. of reads (%) | Median fold coverage (IQR) | No. of reads (%) | Median fold coverage (IQR) | No. of reads (%) | Median fold coverage (IQR) |
| Chromosome | 5,394 | 3,266 (22.32) | 2.0 (1.0–3.0) | 137,068 (11.60) | 4.0 (3.0–6.0) | 2,195,476 (97.70) | 79.0 (67.0–89.0) |
| pKPN-498 | 243 | 3,240 (22.14) | 53.0 (44.0–61.0) | 153,196 (12.97) | 110 (89.0–129.0) | 30,754 (1.37) | 21.0 (17.0–26.0) |
| pKpQIL-6e6 | 113 | 7,566 (51.71) | 252 (215–284) | 493,226 (41.75) | 798 (692.0–884.0) | 10,492 (0.47) | 17.0 (14.0–21.0) |
| pAAC154-a50 | 15 | 401 (2.74) | 54.0 (46.0–63.0) | 393,641 (33.32) | 4633 (3731–5292) | 5,195 (0.23) | 63.0 (51.0–74.0) |
| Unmapped | 159 (1.09) | 4,234 (0.36) | 5,182 (0.23) | ||||
a
Note that some reads mapped ambiguously to multiple replicons due to shared elements. IQR, interquartile range. The accession numbers for KPNIH are NZ_CP008827 (chromosome), NZ_CP008829 (pKPN-498), NZ_CP008830 (pKpQIL-6e6), and NZ_CP008828 (pAAC154-a50).