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. 2017 Nov 27;8:1801. doi: 10.1038/s41467-017-01562-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Endogenous uc.339 sequesters miRNAs. a qRT-PCR for miR-339, -663b, and -95 in A549, H460, H1299, and LoVo cells infected with LV-uc.339 or LV-E, in which uc.339 has been immunoprecipitated. The expression of miRNAs is presented as normalized to the LV-E group. Data are shown as mean ± s.d. of experiments conducted in triplicate. *P < 0.05. b Sequencing of clones #18, #20 and #102 in H1299 cells engineered with CRISPR/Cas9 genomic technology for the deletion of the binding site of miR-339 (nucleotides inside the black box) in the sequence of uc.339. c qRT-PCR for uc.339, miR-339, -663b, and -95 in the immunoprecipitate of uc.339 in H1299 cells wild-type (clone 18) or in clones #20 and #102 of H1299 in which the MBE for miR-339 has been deleted with CRISPR/Cas9 technology. The expression of the RNAs is presented as normalized to the clone 18 group. Data are shown as mean ± s.d. of experiments conducted in triplicate. *P < 0.05. d Cell viability assay in H1299 cells expressing wild-type uc.339 (H1299 CTRL Clone 18) or in CRISPR clones 20 and 102 after 72 h in culture. Data are presented as mean ± s.d. of experiments conducted in triplicate and normalized to H1299 wt. *P < 0.05