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. 2017 Nov 27;7:16365. doi: 10.1038/s41598-017-16555-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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FAD104 expression is elevated during TGF-β–mediated EMT in NMuMG cells. NMuMG cells were treated with 5 ng/mL TGF-β1 or vehicle for 48 h. (A) Morphological changes in NMuMG cells after treatment with TGF-β1. F-actin was visualized by TRITC-conjugated phalloidin. (B) Expression of the epithelial marker ZO-1 and mesenchymal marker N-cadherin after treatment with TGF-β1. Whole-cell lysates were subjected to Western blot analysis and β-actin was used as a loading control. (C) qPCR analysis of fad104 expression in NMuMG cells treated with TGF-β1. The expression level of fad104 was normalized with 18 S rRNA expression. Each column represents the mean with standard deviation (n = 3). Significant differences are indicated as **p < 0.01. (D) Protein expression of FAD104 in NMuMG cells after treatment with TGF-β1. Whole-cell lysates were subjected to Western blot analysis and β-was used as a loading control.