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. 2017 Nov 27;7:16365. doi: 10.1038/s41598-017-16555-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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FAD104 overexpression attenuates TGF-β–mediated EMT in HeLa cells. (A) The effect of FAD104 overexpression on the expression level of EMT-related genes. HeLa cells were infected with FAD104 or LacZ, and treated with 1 ng/mL TGF-β1 for 72 h. Whole-cell lysates were subjected to Western blot analysis and β-actin was used as a loading control. Signal intensities of the proteins were quantified using NIH-Image software. Each column represents the mean with standard deviation (n = 3). Significant differences are indicated as **p < 0.01. (B) Flag-tagged FAD104 expression plasmid was introduced into HeLa cells. After transfection, the cells were treated with 5 ng/mL TGF-β1 for 72 h. The signals of F-actin (red) and Flag-tagged FAD104 (green) were detected with fluorescence microscopy. Arrow indicates the cells expressing Flag-tagged FAD104. Arrowheads indicate untransfected cells as Flag signal is not detected.