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. 2017 Nov 27;7:16365. doi: 10.1038/s41598-017-16555-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Knockdown of fad104 expression enhances the migration of HeLa cells undergoing TGF-β–mediated EMT. HeLa cells were transfected with siRNA targeting fad104 (sifad104-A) and treated with 1 ng/mL TGF-β1 for 72 h. (A) Whole-cell lysates prepared from HeLa cells undergoing EMT were subjected to Western blot analysis. β-Actin was used as a loading control. Arrowhead shows nonspecific bands. (B) Cells undergoing EMT were plated in the upper chamber of the filters coated with fibronectin. Cells that migrated to the underside of the transwell insert were measured after 24 h. Representative images of migrated cells were shown. (C) The mean number of migrated cells in the field was calculated. Each column represents the mean with standard deviation (n = 5). (D) Cells undergoing EMT were plated in culture plates. After 24 h, cells were trypsinized and counted. Each column represents the mean with standard deviation (n = 3). Significant differences are indicated as **p < 0.01 and *p < 0.05.