Figure 3.

Analogue 5d attenuated MMP decrease and attenuated H₂O₂-induced intracellular ROS generation. (A) Representative fluorescent images of JC-1 with analogue 5d in the presence H₂O₂ were shown in the change of mitochondrial membrane high potential (red) and low potential (green). (B) The mitochondrial membrane potential was quantitatively determined with JC-1. (C) The cells were treated with analogue 5d for 12 h before H₂O₂ exposure for 1 h, ROS fluorescence was taken photos. The data are expressed as the means ± S.D. from three independent experiments. ^###P < 0.001 vs. control group; ^***P < 0.001 vs. H₂O₂-treated cells.