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. 2017 Nov 23;8:862. doi: 10.3389/fphar.2017.00862

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Copyright © 2017 Tian, Du, Shang, Wang, Sun, Wang, Deng, Wang, Xu, Sun and Sun.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of analogue 5d on apoptotic-related protein expression and activity. Proteins related to apoptotic signaling were analyzed using Western blotting. (A) Procaspase-3/Cleaved caspase-3 ratio; (B) Western blot analysis of Procaspase-3 and Cleaved caspase-3; (C) Bcl-2/Bax ratio; (D) Western blot analysis of Bcl-2 and Bax; (E) PARP-1/Cleaved PARP-1 ratio; (F) Western blot analysis of PARP-1 and Cleaved PARP-1; β-Actin expression was examined as the protein loading control. The data are presented as the means ± S.D. from three independent experiments. ^##P < 0.01 vs. control group; ^###P < 0.001 vs. control group; ^*P < 0.05 vs. H₂O₂-treated cells; ^**P < 0.01 vs. H₂O₂-treated cells; ^***P < 0.001 vs. H₂O₂-treated cells.