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. 2017 Nov 27;7:16429. doi: 10.1038/s41598-017-16771-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Accumulation of tetradesmethyl MG (m/z 273.14) during MG degradation by free and immobilized laccases at 30 and 60 °C. Tetradesmethyl MG (m/z 273.14) was detected with LC-TOF MS. The highest level of tetradesmethyl MG (m/z 273.14) was observed in MG treated with free laccase at 60 °C for 6 h and was set to 100%. For the laccase treatments of MG, the first letters “F” and “C” indicate free laccase and CLEAs, respectively. The number after the first letter indicates the treatment temperature, and the number after “−” indicates treatment time. After treating MG with free laccase at 60 °C for 6 h, the solution was supplemented with free laccase (F60+F) or CLEAs (F60+C) and incubated overnight.