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Release of antisense strands of double helices by high-affinity anti-DNA mAbs. (A) dsDNA microbead binding assay. Streptavidin-coated microbeads immobilized with double stranded oligonucleotides (5′ biotinylated sense [5′-TATAGTGAGTC-3′] and 5′ FITC-conjugated complementary antisense) were incubated with control human IgG (B, 2 μg/ml), 71F12 (C), or 121G9 (D). Representative results from three independent experiments are shown.
