Fig. 3.

Stressors that modulate histone levels elevate isw-1 expression. a Different RNAi regimens tested in lifespan experiments. (i) RNAi treatment initiated within L1–L3 larvae of previous generation (P0) used for experiment (F1) or (ii) within L1 larvae of generation used for experiment (F1). With regimen (iii), only P0 generation was treated with RNAi, and generation used for experiment (F1) was grown on plates with bacteria expressing empty vector. b Bar graph depicting N2 lifespan treated with RNAi against selected chromatin remodelers according to the RNAi regimen i. See Supplementary Table 2 for lifespan statistics. c–e gfp expression in isw-1 transcriptional reporter (isw-1p::gfp) after c mitochondrial and histone stress, d RNAi of UPRmt core regulators and e ER−, heat or osmotic stress. Experiments with isw-1p::gfp reporter strain were repeated two to three times (N = 12–18). Scale bars, 100 μm. f The effect of ER−, heat or osmotic stress on histone H2A, H2B, and H3 protein levels. Results shown are from three biological replicates