Fig. 5.

ISW-1 overexpression increases lifespan and the expression of sHSPs. a isw-1 expression in isw-1 OE strain. b Chaperone expression in isw-1 OE strain. In b, worms were collected for qRT-PCR analysis at L4 stage. In a and b, bars represent mRNA levels relative to N2 worms treated with empty vector with error bars indicating mean ± s.d. of three biological replicates, each with three technical replicates (*P < 0.05, **P < 0.01; unpaired Student’s t-test). c N2 and isw-1 OE strain lifespan. d N2 and isw-1 OE strain lifespan on sHSP RNAi. See Supplementary Table 2 for lifespan statistics